{"id":13482,"date":"2024-12-20T18:47:35","date_gmt":"2024-12-20T15:17:35","guid":{"rendered":"https:\/\/ap-rad.com\/product\/ehrlichs-reagent-kit\/"},"modified":"2024-12-20T18:47:35","modified_gmt":"2024-12-20T15:17:35","slug":"ehrlichs-reagent-kit","status":"publish","type":"product","link":"https:\/\/ap-rad.com\/en\/product\/ehrlichs-reagent-kit\/","title":{"rendered":"Ehrlich\u2019s Reagent Kit"},"content":{"rendered":"<h1><b>Ehrlich Test<\/b><\/h1>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d><span class=\u201cs2\u201d><b>The Ehrlich test<\/b><\/span> is a biochemical assay used for the <span class=\u201cs2\u201d><b>detection of the amino acid tryptophan<\/b><\/span> and indole-containing compounds in various samples. This test is also known as the <span class=\u201cs2\u201d><b>p-Dimethylaminobenzaldehyde (p-DMAB) test<\/b><\/span>, derived from the main reagent used in the Ehrlich reagent.<\/p>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>This test is named after <span class=\u201cs2\u201d><b>Paul Ehrlich<\/b><\/span>, the Nobel Prize laureate, who originally developed it to <span class=\u201cs2\u201d><b>differentiate typhoid fever from simple diarrhea<\/b><\/span>.<\/p>\n<hr \/>\n<h3><b>Applications of the Ehrlich Reagent Kit<\/b><\/h3>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>The Ehrlich test is considered a <span class=\u201cs2\u201d><b>specific assay<\/b><\/span> for detecting indole-containing compounds and has applications in various laboratory fields, including:<\/p>\n<ul>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Detection of <span class=\u201cs1\u201d><b>tryptophan<\/b><\/span> in protein samples<\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Differentiation of <\/span><b>typhoid fever from non-infectious diarrhea<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Detection of <\/span><b>urobilinogen in urine<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Assistance in diagnosing <\/span><b>liver diseases<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Evaluation of <\/span><b>Carcinoid syndrome<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Identification of <span class=\u201cs1\u201d><b>hemolytic processes<\/b><\/span><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Detection of <\/span><b>biliary tract obstruction<\/b><\/p>\n<\/li>\n<\/ul>\n<hr \/>\n<h3><b>Rapid Ehrlich Test (Spot Test)<\/b><\/h3>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>One common form of the Ehrlich test is the <span class=\u201cs2\u201d><b>spot test<\/b><\/span>, which is used for detecting certain <span class=\u201cs2\u201d><b>psychoactive compounds<\/b><\/span>, including:<\/p>\n<ul>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Tryptamines<\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Ergoloids such as <span class=\u201cs1\u201d><b>LSD<\/b><\/span><\/p>\n<\/li>\n<\/ul>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>It should be noted that <span class=\u201cs2\u201d><b>natural opium<\/b><\/span> shows a <span class=\u201cs2\u201d><b>positive Ehrlich test<\/b><\/span> due to the presence of tryptophan-containing compounds.<\/p>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>The Ehrlich reagent is also used for detecting the following compounds:<\/p>\n<ul>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><b>Indole<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><b>Urobilinogen<\/b><span class=\u201cs1\u201d> present in urine<\/span><\/p>\n<\/li>\n<\/ul>\n<hr \/>\n<h3><b>Objectives of the Ehrlich Test<\/b><\/h3>\n<ul>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Detection of <span class=\u201cs1\u201d><b>tryptophan<\/b><\/span> in the sample<\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Differentiation of <\/span><b>typhoid fever from diarrhea<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Detection of <\/span><b>urobilinogen in urine<\/b><\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>Identification of indole-containing compounds<\/p>\n<\/li>\n<\/ul>\n<hr \/>\n<h3><b>Principle (Mechanism) of the Ehrlich Test<\/b><\/h3>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>The Ehrlich reagent consists of <span class=\u201cs2\u201d><b>p-Dimethylaminobenzaldehyde (p-DMAB)<\/b><\/span>. The reaction is based on <span class=\u201cs2\u201d><b>electrophilic substitution<\/b><\/span> under acidic conditions.<\/p>\n<p class=\u201cp3\u201d dir=\u201crtl\u201d>In this process:<\/p>\n<ul>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>The reagent reacts with the <span class=\u201cs1\u201d><b>indole ring<\/b><\/span> present in tryptophan or its derivatives (such as benzylpyrrole)<\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d>As a result, a <span class=\u201cs1\u201d><b>purple\u2013blue condensation product<\/b><\/span> is formed<\/p>\n<\/li>\n<li>\n<p class=\u201cp1\u201d dir=\u201crtl\u201d><span class=\u201cs1\u201d>Addition of <\/span><b>sodium nitrite (NaNO\u2082)<\/b><span class=\u201cs1\u201d> leads to <\/span><b>intensification of the reaction color<\/b><\/p>\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<ul>\n<li>High sensitivity and detection capability<\/li>\n<li>Includes a Job Aid for ease of use by the operator<\/li>\n<li>Competitive in quality and price compared to leading brands<\/li>\n<\/ul>\n","protected":false},"featured_media":13481,"comment_status":"closed","ping_status":"closed","template":"","meta":{"inline_featured_image":false,"en_name":"","footnotes":""},"product_brand":[],"product_cat":[2486,2487],"product_tag":[2655,2566,2654,2683,2565,2567,3092,3099,3100,3102,3106,3095,3094,3096,3098,3097,3108,3093,3104,3103,3101,3105,3107],"class_list":{"0":"post-13482","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-general-kits","7":"product_cat-urine-chemistry","8":"product_tag-amir-peyvand","9":"product_tag-amirpeyvand","10":"product_tag-ap-rad-2","11":"product_tag-ap-rad-3","12":"product_tag-ap-rad","13":"product_tag-aprad","14":"product_tag-ehrlichs-aldehyde-reagent-for-urine-urobilinogen","15":"product_tag-ehrlichs-aldehyde","16":"product_tag-ehrlichs-aldehyde-kit","17":"product_tag-ehrlichs-aldehyde-reagent","18":"product_tag-ehrlichs-aldehyde-reagent-kit","19":"product_tag-erlich","20":"product_tag-erlichs-reagent","21":"product_tag-erlitch","22":"product_tag-urine-test","23":"product_tag-urine-urobilinogen","24":"product_tag-urine-urobilinogen-reagent-kit","25":"product_tag-urine-urobilinogen-test","26":"product_tag-urine-urobilinogen-test-kit","27":"product_tag-urobilinogen-detection-kit","28":"product_tag-urobilinogen-reagent","29":"product_tag-urobilinogen-test-kit","30":"product_tag-urobilinogen-test-kit-ready-to-use","31":"pa_certificates-ruo","32":"pa_rxn-50ml","34":"first","35":"instock","36":"sold-individually","37":"shipping-taxable","38":"product-type-simple"},"yoast_head":"<!-- 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