IMED License

Borrelia (Burgdorferi) Molecular Detection Kit

Features:

  • Results obtained in the shortest possible time (90 minutes)
  • Detection of various Borrelia species and differentiation of B. burgdorferi species
  • 100% diagnostic sensitivity and specificity
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test

Description

Borrelia Molecular Detection Kit | Borrelia species / B. burgdorferi Real-Time PCR Kit

Borrelia is a genus of Gram-negative spirochete bacteria that causes Borreliosis in humans and animals. Among the various species of this genus, Borrelia burgdorferi is the most well-known pathogenic species in humans and is the primary cause of Lyme Disease.

Transmission of Borrelia bacteria occurs mainly through the bite of hard-bodied ticks (Ixodidae family), particularly black-legged ticks or deer ticks. This infection is more prevalent in wooded and grassy areas.

Lyme disease consists of three clinical stages:

  1. Early stage (Flu-like stage): Mild fever, headache, and a characteristic skin rash called erythema chronicum migrans that appears as a bull’s-eye circular lesion.

  2. Second stage (Neurological symptoms): Involvement of the central and peripheral nervous systems.

  3. Third stage (Joint manifestations): Onset of joint pain and inflammation, particularly in the knees.

Treatment of Lyme disease is typically performed with appropriate antibiotics. However, a small percentage of patients may experience symptoms such as chronic fatigue, muscle or joint pain months or years after treatment, known as Post-Treatment Lyme Disease Syndrome, which does not indicate active infection.


Introduction of Borrelia species / B. burgdorferi Real-Time PCR Kit

ap-rad, utilizing Real-Time PCR technology and specific primer and probe design, has developed the Borrelia species / B. burgdorferi Real-Time PCR Kit for rapid, sensitive, and differential detection of various Borrelia species and specific differentiation of Borrelia burgdorferi in human samples.

This kit can accurately detect bacterial DNA in the early stages of infection and is highly suitable for use in molecular diagnostic laboratories, research centers, and epidemiological studies of tick-borne diseases.

اطلاعات کیت
Description Real-Time Amplification Kit with Bacterial DNA Extraction of Specimen
Method Real-Time
Type Qualitative
Regulatory Status RUO
Storage & Transfer Condition (˚C) 20-
Number of Reactions 25
Controls Positive & Negative
Applicable Instruments All Type of Real-Time PCR Machines with FAM, JOE and ROX Channels
Result Display Time 1.5hours
Validated Specimens Peripheral Blood
CSF
Sample Volume 5µL
Analytical Sensitivity (Copy/µL)s 1.8Copy/µL
Analytical Specificity 100%
Clinical Sensitivity 100%
Clinical Specificity 100%
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REF: AP10STIK1
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Detection Capability:

  • Mycoplasma (M. hominis / M. genitalium)
  • Chlamydia trachomatis
  • Ureaplasma (U. urealyticum / U. parvum)
  • Herpes simplex virus 2 (HSV-2)
  • Herpes simplex virus 1 (HSV-1)
  • Trichomonas vaginalis
  • Treponema pallidum
  • Neisseria gonorrhoeae
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REF: AP11BFRK1
RUO

Detection of 10 bacteria including:

  • Streptococcus pneumoniae
  • Staphylococcus aureus
  • Haemophilus influenzae
  • Mycoplasma pneumoniae
  • Chlamydia pneumoniae
  • Legionella pneumophila
  • Pseudomonas aeruginosa
  • Klebsiella pneumoniae
  • Bordetella pertussis
  • Bordetella parapertussis

and one fungus including:

  • Pneumocystis jirovecii
6-Plex Bacterial Meningitis
REF: AP6PBMRK1
RUO

6-Plex Bacterial Meningitis-AP6PBMRK1 includes:

  • Streptococcus pneumoniae
  • Neisseria meningitidis
  • Haemophilus influenzae
  • Escherichia coli
  • Listeria monocytogenes
  • Streptococcus agalactiae (GBS)
5 Bacterial Pneumonia Detection Real Time PCR Kit
REF: AP5PBPRK1
RUO

Detection capability for five pneumonia bacteria including:

  • Streptococcus pneumoniae
  • Staphylococcus aureus
  • Haemophilus influenzae
  • Klebsiella pneumoniae
  • Pseudomonas aeruginosa
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REF: APRUBVK1
RUO
  • Results obtained in the shortest possible time (2 hours)
  • 100% diagnostic sensitivity and specificity
  • Performed in a one-step reaction (no need for cDNA synthesis)
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test
Placeholder
REF: APMEAVK1
RUO
  • Results obtained in the shortest possible time (2 hours)
  • 100% diagnostic sensitivity and specificity
  • Performed in a one-step reaction (no need for cDNA synthesis)
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test