IMED License

Brucella Molecular Detection Kit

Features:

  • Results obtained in the shortest possible time (90 minutes)
  • Detection of various Brucella species and differentiation of Brucella melitensis and Brucella abortus species in two reactions
  • 100% diagnostic sensitivity and specificity
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test

Description

Brucella Real-Time PCR Kit

Brucella are Gram-negative, small, aerobic, and non-motile bacteria that cause brucellosis (Malta fever, undulant fever, or Mediterranean fever) in humans and animals, particularly livestock.

This genus includes 12 species, with Brucella melitensis and Brucella abortus being the most pathogenic species for humans.

The incubation period from the moment of contact with the infection source (such as dairy products, raw meat from infected livestock, or through airborne transmission) until symptom onset is typically between 1 to 3 weeks, and in some conditions may extend up to 6 months. After symptom onset, the disease is divided into three stages based on severity: acute, subacute, and chronic.

Symptoms of brucellosis include:

  • Sudden chills
  • Generalized body pain, particularly in the back
  • Profuse sweating, loss of appetite, weakness, and fatigue
  • Recurrent fevers
  • In the chronic phase: arthritis, endocarditis, and spondylitis

This disease can also affect the central nervous system, liver, heart, and fertility of the infected individual.


Introduction of Brucella Real-Time PCR Kit

ap-rad, utilizing Real-Time PCR technology and specific primer and probe design, has developed the Brucella Real-Time PCR Kit for rapid, sensitive, and specific detection of various Brucella species and simultaneous differentiation of Brucella melitensis and Brucella abortus.

This kit is suitable for molecular diagnostic laboratories, research centers, and infectious disease control centers and enables accurate detection of bacterial DNA in clinical samples.

اطلاعات کیت
Description Real-Time Amplification Kit with Bacterial DNA Extraction of Specimen
Method Real-Time
Type Qualitative
Regulatory Status RUO
Storage & Transfer Condition (˚C) 20-
Number of Reactions 25 & 50
Controls Positive & Negative
Applicable Instruments All Type of Real-Time PCR Machines with FAM, JOE and ROX Channels
Result Display Time 1.5hours
Validated Specimens Peripheral Blood
Sample Volume 10µL
Analytical Sensitivity (Copy/µL)s 12 (Brucella species), 8.7 (Brucella melitensis) and 8.5 (Brucella abortus) Copy/µL
Analytical Specificity 100%
Clinical Sensitivity 100%
Clinical Specificity 100%
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Detection Capability:

  • Mycoplasma (M. hominis / M. genitalium)
  • Chlamydia trachomatis
  • Ureaplasma (U. urealyticum / U. parvum)
  • Herpes simplex virus 2 (HSV-2)
  • Herpes simplex virus 1 (HSV-1)
  • Trichomonas vaginalis
  • Treponema pallidum
  • Neisseria gonorrhoeae
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Detection of 10 bacteria including:

  • Streptococcus pneumoniae
  • Staphylococcus aureus
  • Haemophilus influenzae
  • Mycoplasma pneumoniae
  • Chlamydia pneumoniae
  • Legionella pneumophila
  • Pseudomonas aeruginosa
  • Klebsiella pneumoniae
  • Bordetella pertussis
  • Bordetella parapertussis

and one fungus including:

  • Pneumocystis jirovecii
6-Plex Bacterial Meningitis
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6-Plex Bacterial Meningitis-AP6PBMRK1 includes:

  • Streptococcus pneumoniae
  • Neisseria meningitidis
  • Haemophilus influenzae
  • Escherichia coli
  • Listeria monocytogenes
  • Streptococcus agalactiae (GBS)
5 Bacterial Pneumonia Detection Real Time PCR Kit
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Detection capability for five pneumonia bacteria including:

  • Streptococcus pneumoniae
  • Staphylococcus aureus
  • Haemophilus influenzae
  • Klebsiella pneumoniae
  • Pseudomonas aeruginosa
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  • Results obtained in the shortest possible time (2 hours)
  • 100% diagnostic sensitivity and specificity
  • Performed in a one-step reaction (no need for cDNA synthesis)
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test
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  • Results obtained in the shortest possible time (2 hours)
  • 100% diagnostic sensitivity and specificity
  • Performed in a one-step reaction (no need for cDNA synthesis)
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test