Brucella Real-Time PCR Kit

Features:

  • Results obtained in the shortest possible time (90 minutes)
  • Detection of various Brucella species and differentiation of Brucella melitensis and Brucella abortus species in two reactions
  • 100% diagnostic sensitivity and specificity
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test

Description

Brucella Real-Time PCR Kit

Brucella are Gram-negative, small, aerobic, and non-motile bacteria that cause brucellosis (Malta fever, undulant fever, or Mediterranean fever) in humans and animals, particularly livestock.

This genus includes 12 species, with Brucella melitensis and Brucella abortus being the most pathogenic species for humans.

The incubation period from the moment of contact with the infection source (such as dairy products, raw meat from infected livestock, or through airborne transmission) until symptom onset is typically between 1 to 3 weeks, and in some conditions may extend up to 6 months. After symptom onset, the disease is divided into three stages based on severity: acute, subacute, and chronic.

Symptoms of brucellosis include:

  • Sudden chills
  • Generalized body pain, particularly in the back
  • Profuse sweating, loss of appetite, weakness, and fatigue
  • Recurrent fevers
  • In the chronic phase: arthritis, endocarditis, and spondylitis

This disease can also affect the central nervous system, liver, heart, and fertility of the infected individual.


Introduction of Brucella Real-Time PCR Kit

ap-rad, utilizing Real-Time PCR technology and specific primer and probe design, has developed the Brucella Real-Time PCR Kit for rapid, sensitive, and specific detection of various Brucella species and simultaneous differentiation of Brucella melitensis and Brucella abortus.

This kit is suitable for molecular diagnostic laboratories, research centers, and infectious disease control centers and enables accurate detection of bacterial DNA in clinical samples.

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  • Results obtained in the shortest possible time (2 hours)
  • 100% diagnostic sensitivity and specificity
  • Performed in a one-step reaction (no need for cDNA synthesis)
  • Contains internal, positive, and negative controls to confirm the extraction process and PCR test
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