Description
MTC and NTM Multiplex PCR Kit (Real‑time)
Amir Peyvand Research and Development Company (AP‑RAD) has developed the MTC and NTM Multiplex PCR Kit (Real‑time) with the aim of providing rapid, sensitive, and highly specific detection of Mycobacterium tuberculosis complex (MTC) and Nontuberculous Mycobacteria (NTM) in clinical specimens such as sputum, bronchoalveolar lavage (BAL), body fluids, and FFPE tissue samples from patients with symptoms or suspected mycobacterial infections.
This kit leverages advanced multiplex PCR technology, enabling accurate and fast identification of these bacteria.
Mycobacteria
Mycobacteria are responsible for various diseases, most notably tuberculosis (TB), which remains a major global health concern and contributes to significant mortality worldwide. In recent years, the global prevalence of nontuberculous mycobacteria (NTM) has markedly increased in both developed and developing countries.
Clinically, NTM infections are often indistinguishable from TB, creating diagnostic challenges and complicating treatment—especially in patients undergoing long‑term therapy for pulmonary TB.
Because different mycobacterial species vary in pathogenicity and antibiotic susceptibility, early and accurate species‑level identification is essential for proper patient management and infection control. This need is even more critical in cases involving drug‑resistant infections.
Intended Use
The Qualitative Multiplex PCR Kit for NTM & MTC from AP‑RAD is designed for use by qualified clinical and research laboratories experienced in real‑time PCR techniques and molecular diagnostic workflows.
The kit features a user‑friendly protocol that minimizes handling steps and reduces the risk of error while ensuring high diagnostic accuracy. All necessary PCR components are included, facilitating a faster and more streamlined testing process.
Using this kit, laboratories can rapidly and reliably detect both Mycobacterium tuberculosis complex and Nontuberculous Mycobacteria in a wide variety of specimens. Early detection supports timely and appropriate therapeutic decisions and contributes to more effective control and prevention of mycobacterial infections.
Produced according to international standards and utilizing advanced molecular technology, the MTC and NTM Multiplex PCR Kit is engineered to meet the evolving needs of clinical and research laboratories by significantly improving diagnostic speed and precision.
Key Features
- Qualitative multiplex detection of NTM & MTC compatible with three‑channel real‑time PCR instruments commonly used in Iran, including ABI StepOne Plus
- Fully tested and calibrated on instruments such as:Rotor‑Gene Q 5plex Platform, Rotor‑Gene Q 5plex HRM Platform, Rotor‑Gene Q 2plex / 6plex (Qiagen), ABI StepOne, ABI StepOne PlusChannels supported: FAM (green), JOE/VIC/HEX (yellow), ROX (orange)
- Utilizes RNase P gene as an internal control
- No reagent preparation required
- Excellent kit stability
