Description
PML::RARA Fusion Detection rRT-PCR Kit (Real-time) | PML::RARA Fusion Detection Kit
The chromosomal translocation t(15;17), which results in the fusion of the PML gene on chromosome 15 with the RARA gene on chromosome 17, is the cause of Acute Promyelocytic Leukemia (APL). APL is a subtype of Acute Myeloid Leukemia (AML – morphology M3) and is considered one of the most dangerous types of leukemia because it is associated with severe coagulation disorders and, without rapid diagnosis, can lead to early patient death.
Rapid and accurate diagnosis of this disease plays a vital role in reducing mortality; as timely initiation of specific treatments such as ATRA and arsenic trioxide can transform APL from a potentially fatal condition into one of the most treatable types of leukemia.
The PML::RARA fusion can produce three different isoforms depending on the breakpoint location in the PML gene:
- bcr1 (PML::RARA L – Long): Breakpoint in intron 6
- bcr2 (PML::RARA V – Variant): Breakpoint in exon 6
- bcr3 (PML::RARA S – Short): Breakpoint in intron 3
Accurate identification of these isoforms holds great value in molecular interpretation, disease monitoring (MRD), and treatment decision-making.
ap-rad, utilizing proprietary design and Real-Time PCR technology, has produced the PML::RARA Fusion Detection rRT-PCR Kit. This kit is designed and manufactured with the following objectives:
- Rapid, sensitive, and specific detection of the PML::RARA fusion
- Precise differentiation of the three isoforms bcr1, bcr2, and bcr3
- Application in initial diagnosis, molecular confirmation of APL, and monitoring treatment response
It is considered a reliable tool for specialized molecular diagnostic laboratories in the field of hematology.
